TABLE 3.
Category and locusb | Gene | Function | TPM in WT | TPM in WT(k) | Fold change [WT(k)/WT] | P value | TPM in Δccg-8 | TPM in Δccg-8(k) | Fold change [Δccg-8(k)/Δccg-8] | P value | Fold change [Δccg-8(k)/WT(k)] | Sensitivity to ketoconazole compared to WT's |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Upregulated genes | ||||||||||||
NCU09691 | kts-3 | Hypothetical protein | 0.01 | 14.83 | 1483 | 8.88E−16 | 0.01 | 3.87 | 387 | 0.00012 | 0.26 | Hypersensitive |
NCU02084 | Arginase | 13.82 | 1007.43 | 72.90 | 9.08E−14 | 67.92 | 304.5 | 4.48 | 2.78E−13 | 0.30 | Hypersensitive | |
NCU09635 | kts-4 | Hypothetical protein | 3.17 | 216.04 | 68.15 | 7.57E−07 | 8.3 | 69.88 | 8.42 | 2.00E−11 | 0.32 | Hypersensitive |
NCU01315 | gt41-1 | UDP-N-acetylglucosaminyltransferase | 2.59 | 38.9 | 15.02 | 2.57E−06 | 20.46 | 11.3 | 0.55 | 0.002807 | 0.29 | Hypersensitive |
NCU08957 | kts-5 | Hypothetical protein | 5.47 | 69.4 | 12.69 | 4.61E−09 | 16.31 | 27.06 | 1.66 | 0.002712 | 0.39 | Hypersensitive |
NCU01633 | hxt13 | Hexose transporter HXT13 | 377.04 | 4679.24 | 12.41 | 1.73E−12 | 622.5 | 1352.73 | 2.17 | 0 | 0.29 | Hypersensitive |
NCU00247 | kts-6 | Hypothetical protein | 6.62 | 64.36 | 9.72 | 3.39E−10 | 7.71 | 13.98 | 1.81 | 0.013643 | 0.22 | Hypersensitive |
NCU01302 | Hydrolase | 8.63 | 41.98 | 4.86 | 3.78E−12 | 17.2 | 9.81 | 0.57 | 0.009111 | 0.23 | Hypersensitive | |
NCU00611 | kts-7 | Hypothetical protein | 8.06 | 30.78 | 3.82 | 1.54E−11 | 6.52 | 14.57 | 2.23 | 0.001244 | 0.47 | Hypersensitive |
NCU00248 | kts-8 | Hypothetical protein | 59 | 216.04 | 3.66 | 3.92E−13 | 92.24 | 85.05 | 0.92 | 0.322136 | 0.39 | Hypersensitive |
Downregulated genes | ||||||||||||
NCU04334 | cpn10 | Chaperonin | 45.76 | 14.55 | 0.32 | 1.18E−14 | 18.09 | 31.22 | 1.73 | 0.000581 | 2.15 | Resistant |
NCU04923 | gcy-1 | Glycerol dehydrogenase | 83.76 | 33.86 | 0.40 | 1.77E−18 | 462.4 | 163.85 | 0.35 | 1.7E−110 | 4.84 | Resistant |
WT, wild type; WT(k) or Δccg-8(k), wild type or Δccg-8 mutant treated with ketoconazole, respectively; TPM, number of locus tags per million; NA, the mutant was not available.
Locus numbers and function were annotated according to the N. crassa genome assembly (http://www.broadinstitute.org/annotation/genome/neurospora/).