FIG 3.

LmaCK1.2 is sensitive to known CK1 inhibitors. (A) Kinase assay using 27 μM CK-S and 1 μg recombinant LmaCK1.2 in the presence of either 1% DMSO alone or D4476, IC261, and CKI-7 at 10 μM in 1% DMSO. (B) Kinase assay using 36 μg dephosphorylated casein and 1 μg of recombinant LmCK1.2 in the presence of either DMSO or inhibitors at 10 μM (as described for panel A). (a) Autoradiography; (b) Coomassie staining. (C) Determination of the IC₅₀ of D4476 (a), CKI-7 (b), and IC261 (c) for mammalian CK1 from porcine brain (SsCK1) and recombinant LmaCK1.2. We performed kinase assays using SsCK1 or LmaCK1.2 in the presence of increasing concentrations of the respective CK1 inhibitors.