FIG 2.

Diagrams for HRV replicon constructs. Replicons with identical gene arrangements were built for HRV-C genotypes HRVc15, HRVc11, HRVc24, and HRVc25 and for the species A genotype HRV16. Full-length constructs were made using the coding sequences for HRVc15 and HRV16. All constructs contain a T7 promoter at the 5′ end for in vitro transcription and an ∼50-bp poly(A) tract upstream of MluI site following the 3′ NCR. For the HRV-C replicons, the cis replication element (CRE) normally found in VP2 was moved to the 3′ NCR. HRVc15-RepMut, an HRVc15 replicon containing the 3Dpol N295A substitution, was constructed to assess the replicon baseline luciferase expression and RNA levels.