(A) Schematic depiction of mutated ARM58 constructs used for panel B. The shaded boxes represent the 4 DUF1935 putative domains. The black box signifies the putative transmembrane domain (TMD). The numbers indicate the positions relative to the wild-type amino acid sequence. (B) SbIII IC50s (μM) for L. infantum carrying the indicated ARM58 mutants. *, P < 0.05 (n = 4) relative to full-length ARM58. (C) Schematic depiction of ARM58rel, ARM58, and two DUF1935-III domain swapping mutants, ARM58_3Drel and ARM58rel_D3. (D) As for panel B, but testing domain swapping mutants of ARM58 and AMR58rel (n = 4).