FIG 5.

Intracellular Sb concentration following Sb^III exposure. L. infantum(pCLN) and L. infantum(pCLN-ARM58) were incubated for 48 h with 0 μM or at 400 μM Sb^III. Cells were then counted (A). From each culture, 5 × 10⁷ promastigotes were precipitated, washed, and lysed in nitric acid. Sb concentration in each lysate was measured by inductively coupled plasma mass spectrometry (ICPMS) (n = 3) (B). (C and D) Induction of Sb^III resistance in strains receiving 10 μM verapamil (C) or 50 μM sodium vanadate (D). Vector controls and ARM58 overexpressing L. infantum were grown for 72 h. Cell densities were measured and plotted against the indicated Sb^III concentrations. Values are the medians from 4 independent experiments. V, verapamil; SV, sodium vanadate.