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Primers used in this study
| Primer | Nucleotide sequence (5′-3′) |
|---|---|
| OmpAint up | GTTAAAGGCGACGTAGACG |
| OmpAint lw | CCAGTGTTATCTGTGTGACC |
| OmpAext up | GGAATGGCTATAACTGACATAATC |
| OmpAext lw | GAATCAGGAGATTTACAAATGACC |
| OmpA-EcoRI F | GACTAGGAATTCGGAATGGCTATAACTGACATAATC |
| OmpA-EcoRI R | GACTAGGAATTCGAATCAGGAGATTTACAAATGACC |
| M13-Fa | GTAAAACGACGGCCAGT |
| M13-Ra | CAGGAAACAGCTATGAC |
Primer designed in pGEM-T.
