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Inhibition of alamarBlue reduction by nitrofurans. (A) Wild-type E. coli; (B) ΔnfsA ΔnfsB double mutant (KAN resistant). The strong inhibition of alamarBlue reduction by kanamycin, as shown in panel A, is due to its high concentration (50 µg/ml). The experiment was performed in a microtiter plate, and fluorescence was measured every 10 min with an excitation wavelength of 544 nm and an emission wavelength of 590 nm.
