TABLE 1.
Bacterial strains used in this study
| Strain or species | Relevant genotype | Parent or source | Reference or strain designationd |
|---|---|---|---|
| BW25113 | K-12 rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1 | 15 | |
| nfsB-FRT | ΔnfsB::FRT | JW0567 | 15 |
| MV1970 | ΔnfsB::FRT ΔnfsA::kan | JW0835 into nfsB-FRT | 15 |
| tolC | ΔtolC::kan | JW5503 | 15 |
| acrB | ΔacrB::kan | JW0451 | 15 |
| nfsA+ | BW25113(pZS*24nfsA) | 17 | |
| nfsA− | ΔnfsA::kan | JW0835 | 15 |
| nfsB+ | BW25113(pZS*24nfsB) | 17 | |
| nfsB− | ΔnfsB::kan | JW0567 | 15 |
| Francisella tularensis | SchuS4a | ||
| Bacillus anthracis | Amesa | ||
| Yersinia pestis | KIMa | ||
| Escherichia coli | ATCC 25922 | ||
| Staphylococcus aureus | NCTC 8325 | ||
| Enterococcus faecalis | ATCC 47077 | ||
| Salmonella Typhimurium (LT2) | ATCC 700720 | ||
| Staphylococcus aureus (MRSA)e | NRS54b, Novobiotic Pharmaceuticals | ||
| Clostridium difficile (CD196) | Clinical isolate, CD196c | ||
| Enterococcus faecium (VRE BM4147)f | BM4147, Novobiotic Pharmaceuticals | ||
| Acinetobacter baumannii | ATCC 17978 | ||
| Pseudomonas aeruginosa (PAO1) | ATCC BAA-47 |
a
Strain designation from the NERCE/BEID facility.
b
Strain designation from the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA).
c
This clinical isolate was a gift from Linc Sonenshein at Tufts University.
d
Abbreviations: ATCC, American Type Culture Collection; NCTC, National Collection of Type Cultures.
e
MRSA, methicillin-resistant Staphylococcus aureus.
f
VRE, vancomycin-resistant Enterococcus faecium.