FIG 2.

A. castellanii viability determination by trypan blue exclusion method. Trophozoites were incubated with 200 μM corifungin for different time periods. (A) Amebae with fresh medium only. (B) Amebae incubated with corifungin for 24 h. (C) Amebae incubated with corifungin for 48 h. (D) Amebae incubated with corifungin for 72 h. (E) Amebae incubated with corifungin for 96 h. (F) Amebae incubated with corifungin for 120 h. Cells stained blue were considered nonviable (arrowheads). Magnification, ×60.