TABLE 5.

Median MICs for plasmid-bearing strains versus ceftriaxone

Strain/plasmidb	MIC (µg/ml)a
	− cCF10	+ cCF10c
OG1/vector	32	16
OG1/vanYB	32	4
V583/vector	512	256
V583/vanYB	512	4
V583/vanYB Δ1-46	512	512
V583/vanYB D195A	512	512
V583/vanYB E238A	512	512

^aMICs were determined in MHB supplemented with Cm (no vancomycin) after 24 h at 37°C from a minimum of 3 independent experiments.

^bThe plasmids used were as follows: vector, pBK2; vanY_B, pJLL98; vanY_B Δ-46, pJLL99; vanY_B D195A, pJLL107; vanY_B E238A, pJLL108.

^ccCF10 was used to induce plasmid-borne gene expression at 10 ng/ml for OG1 host strains or at 0.5 ng/ml for V583 host strains.