FIG 7.

Inhibition of TNF-α- and IL-1β-induced NF-κB-dependent gene transcription by vv811ΔA49 does not require the expression of late viral proteins. (a, b) A549-NF-κB-LUC cells were mock infected or infected in quadruplicate for 6 h with 5 PFU per cell of virus either treated (AraC) or not treated (n.t.) with AraC at 40 μg/ml and then stimulated for 6 h with TNF-α (50 ng/ml) (a) or IL-1β (20 ng/ml) (b) or nonstimulated (NS) as a control by incubation with medium alone. The cells were lysed in passive lysis buffer, and the luminescence of each sample was measured and normalized to that for the nonstimulated mock-infected cells to give the fold induction. Data are shown as the mean ± SD and are representative of those from three experimental repeats. (c) Protein expression was analyzed by SDS-PAGE and immunoblotting using antibodies against the indicated proteins. Molecular mass markers (in kDa) are indicated on the right.