TABLE 3.
Types of mutations in the mutant spectrum of ML29 in vivo and in vitroa
| Mutation | No. |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vero cells |
Rhesus monkeys |
Marmosets |
C57 mice |
CBA/J mice |
||||||||||||||||
| GP | NP | Z | L | GP | NP | Z | L | GP | NP | Z | L | GP | NP | Z | L | GP | NP | Z | L | |
| A→C | 1 | 1 | ||||||||||||||||||
| A→G | 7 | 1 | 4 | 4 | 1 | 1 | 1 | |||||||||||||
| A→U | 1 | 1 | 2 | 1 | 1 | |||||||||||||||
| C→A | 1 | 1 | ||||||||||||||||||
| C→G | 1 | 1 | ||||||||||||||||||
| C→U | 1 | 1 | 3 | 4 | 1 | 3 | 1 | 2 | 1 | |||||||||||
| G→A | 2 | 2 | 1 | 2 | 2 | 1 | 1 | 1 | ||||||||||||
| G→C | ||||||||||||||||||||
| G→U | 1 | 1 | ||||||||||||||||||
| U→A | 1 | 1 | ||||||||||||||||||
| U→C | 2 | 4 | 3 | 1 | 2 | 2 | 1 | 3 | 1 | |||||||||||
| U→G | 1 | 1 | 1 | 1 | ||||||||||||||||
| Deletions | 1 | 1 | 1 | 2 | 1 | |||||||||||||||
| Insertions | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||||||||||||
| Noncoding | 1 | 2 | 3 | 1 | 1 | 1 | ||||||||||||||
| Total no. of mutations | 6 | 1 | 10 | 4 | 9 | 1 | 20 | 2 | 6 | 1 | 18 | 1 | 4 | 1 | 7 | 3 | 5 | 1 | 8 | |
| Transitions | 6 | 9 | 2 | 8 | 13 | 4 | 10 | 1 | 2 | 0 | 4 | 3 | 5 | 3 | ||||||
| Transversions | 2 | 1 | 3 | 2 | 1 | 1 | 2 | 1 | 1 | 1 | 3 | |||||||||
| Nonsynonymous | 4 | 3 | 2 | 5 | 1 | 8 | 2 | 2 | 8 | 2 | 1 | 2 | 1 | 3 | 2 | |||||
| Synonymous | 1 | 6 | 2 | 3 | 8 | 3 | 1 | 5 | 1 | 1 | 3 | 1 | 2 | 4 | ||||||
| Selection rate | 4 | 0.5 | 1 | 1.6 | 1 | 0.7 | 1.6 | 2 | 0.7 | 1 | 1.5 | 0.5 | ||||||||
a
The table shows the types of changes observed in each host within each viral protein. The selection rate was obtained from the dN/dS, ratio in which dN represents nonsynonymous and dS synonymous changes at the protein level.