FIG 3.

P58 is a cis-acting protein. (A) Complementation between RNA2 mutants with defects in P58 and MP, as revealed by confocal microscopy of the bombarded cotyledons. The diagrams of the mutants used, as well as those included in each of the complementation sets, are shown on the left, with the outcomes of infections/complementations shown on the right. mCh, red fluorescent mCherry protein. (B) Results of (−) RNA2-specific sqRT-PCR confirming the complementation results shown in panel A. Note that the relative accumulation levels were calculated by using R2G-mMP (top panel) or R2R-mMP (middle panel) as a reference (arbitrarily set as 1).