FIG 4.

Replication-related proteins encoded by RNA1 are all cis-acting. (A) Schematic representation of the constructs used in this set of experiments (top), along with their fates in bombarded lima bean cotyledons (bottom). (B) Confirmation of results shown in panel A by (−) RNA1-specific sqRT-PCR. (C) The defect of RNA1m could not be complemented by functional RdRP provided by wild-type RNA1. Digestion with BsrBI, which cuts the PCR fragment of the R1m mutant but not that of wild-type RNA1 (lanes 1 and 2), was used to detect potential R1m-specific RT-PCR products amplified from RNA1-plus-R1m-2G-cobombarded cotyledons. (D) Schematic representation of five RNA1 mutants tested in this study. The names of the mutants, together with the specific aa changes introduced by these mutants, are highlighted in their corresponding boxes.