FIG 3.
Sedimentation velocity analysis of U21NSBPHA. (a) Representative radial absorbance profiles at 280 nm as a function of time, from an experiment at a loading concentration of 0.26 mg/ml U21NSBPHA in 12-mm path length centerpieces recorded at 280 nm (symbols, with times indicated by the color temperature); best-fit profiles from the c(s) distribution (solid lines). For clarity, only every 3rd data point of every 3rd scan is shown. Residuals of the fit, with an RMSD of 0.0051 OD, are shown in the lower panel. (b) c(s) distribution from the model shown in panel a (purple). Also shown are equivalent distributions obtained at a 2-fold-higher concentration in a 3-mm path length centerpiece recorded at 280 nm (blue) and a 10-fold-lower concentration in a 12-mm centerpiece recorded at 230 nm (green).