FIG 5.
Multisignal sedimentation velocity (MSSV) analysis based on a global fit of data recorded by Rayleigh interference, absorbance at 280 nm and 250 nm, and analyzed in terms of a spectrally and diffusionally deconvoluted component sedimentation coefficient distribution [ck(s)] of HLA-A2 (blue) and U21NSBPHA (red). For ease of comparison, vertical bars denote the sedimentation coefficients of U21 dimers (gray) and tetramers (green), as shown in Fig. 3. (a) Multisignal sedimentation velocity ck(s) analysis of a loading mixture of 39.4 μM HLA-A2 and of 11.8 μM U21NSBPHA. Raw boundary data and global fits are available from the author. (b) ck(s) analysis of a loading mixture of 4.9 μM HLA-A2 and 3.3 μM U21NSBPHA.