Neutralization assays of single- and double-mimetic peptides. (A) HIV-1 pseudotyped with the envelope glycoproteins of the indicated HIV-1 isolates was incubated with TZM-bl cells and with CD4-Fc or the indicated double-mimetic Fc fusions. Infection was determined by luciferase activity 48 h later and is shown as the percentage of luciferase units in the absence of inhibitor. (B) Assay similar to that described for panel A except that CD4mim6-Fc, CCR5mim3-Fc, a mixture of these constructs, Rev-Fc, 16-Fc, and CD4-Fc were assayed. All neutralization studies were performed in triplicate, with bars representing standard errors. Experiments are representative of two with similar results.