FIG 1.

HCV infection modifies consumption and secretion of metabolites in Huh7.5 cells. Replicate cultures of Huh7.5 cells were serum starved for 24 h and subsequently mock infected (control) or HCV infected at an MOI of 1 (infected). Supernatants and cells were harvested at 0, 8, 24, 48, and 72 h postinfection and processed for metabolites and protein quantifications. (A) Protein extracts from cell homogenates at each time point were subjected to Western blotting and stained with anti-Core antibody (clone C7-50). Variations in glucose (B), glutamine (C), pyruvate (D), lactate (E), and glutamate (F) concentrations in cell culture medium were normalized with total protein amounts in the well and are expressed as nmol consumed or secreted (compared to time zero) per μg of protein (Δnmol/μg). Error bars show ± standard errors of the means (n = 3). *, P < 0.01, between infected and control cells (t test). MW, molecular weight (in thousands).