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Expression of hexokinase isoforms in hepatoma cell lines. Total cellular mRNAs were extracted from 1 × 106 Huh7.5, HepG2, and Huh9.13 cells as described in Materials and Methods. Distinct pairs of primers were used to specifically amplify by PCR transcripts of HK1, HK2, HK3, and HK4 before separation on agarose gels. GAPDH transcripts were used for normalization.
