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. 2014 Mar;88(6):3464–3473. doi: 10.1128/JVI.02914-13

FIG 3.

FIG 3

Interaction of FBXO3 with RVFV NSs. (A) Cartoon depicting the domain structure of FBXO3 isoforms (UniProt accession number Q9UK99), drawn by using the image creator MyDomains of the ExPASy website (45). (B) Immunoprecipitation. 293 FLP-IN TREX cells expressing SII-HA-tagged FBXO3/1 or FBXO3/2 were induced with doxycycline for 2 days and then infected with rZH-CF-NSs or rZH-NF-ΔMx (MOI of 3). Three hours p.i., MG132 was added to the medium, cells were incubated for another hour, lysed, and subjected to immunoprecipitation using the indicated antibodies.