FIG 7.

NSs and components of canonical SCF ubiquitin ligases. (A and B) Western blot analysis of knockdown cells. HeLa cells were treated with a control siRNA or with siRNAs directed against mRNAs of cullin 1 (Cul1), FBXO3, Rbx1, or Skp1 (A) or cullin 1 and/or cullin 7 (B). Cells were then infected with recombinant wt RVFV (rZH548) or Clone 13 (Cl13) at an MOI of 10 and analyzed 6 h later for the presence of the indicated proteins. (C) Immunofluorescence analysis. HeLa cells with siRNA knockdown of cullin 1, Rbx1, or Skp1 mRNAs were infected for 6 h with rZH-CF-NSs at an MOI of 3, and the presence of nuclear DNA, p62, and Flag-RVFV NSs was analyzed 6 h later using DAPI stain and appropriate antibodies, respectively.