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Plasma antibodies block the binding of MAbs and sCD4. Plasma antibodies blocked binding of labeled ligands to Env proteins. Binding of sCD4 (A) and MAb b12 (B) to gp140 B.JRFL was inhibited by immune plasma. Titers were lowest for STS and highest for STS+oCpG+R848. (C) Blocking of ADCC-mediating MAb A32 was lowest for STS and highest for STS+R848. (D) Low-level blocking of broadly neutralizing MAb CH01 was found in the STS+oCpG+R848-immunized group.
