TABLE 2.
Distribution of viral infection in various organs as assessed by ISHa
| Groupb | DPIc | No. of ferretsd | No. of ferrets that exhibited influenza virus RNA ine: |
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Trachea | Bronchi | Alveoli | Submucosal glands | Small bowel |
Liver | Spleen |
Brain |
Kidney | |||||||||||
| Epithelia | Lamina propria | Ganglia | Red pulp | White pulp | Olfactory bulb | Cerebral cortex | Deep gray and white matter | Brainstem | Cerebellum | Meninges and ependyma | |||||||||
| Naive | NA | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | NA |
| H1N1 | 2 | 4 | 3 | 3 | 2 | 2 | 0 | 0 | 0 | NA | NA | NA | 0 | 0 | 0 | 0 | 0 | 0 | NA |
| 3 | 4 | 2 | 3 | 1 | 3 | 0 | 2 | 0 | NA | NA | NA | 0 | 0 | 0 | 0 | 0 | 0 | NA | |
| 5 | 4 | 4 | 4 | 2 | 4 | 0 | 0 | 0 | NA | NA | NA | 0 | 0 | 0 | 0 | 0 | 0 | NA | |
| 7 | 4 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | NA | NA | NA | 0 | 0 | 0 | 0 | 0 | 0 | NA | |
| 14 | 4 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | NA | NA | NA | 0 | 0 | 0 | 0 | 0 | 0 | NA | |
| H5N1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | NA |
| 4 | 2 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | NA | |
| 5 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | NA | |
| 6 | 4 | 1 | 0 | 3 | 0 | 0 | 1 | 0 | 4 | 0 | 2 | 2/3 | 2/3 | 2/3 | 3/3 | 3/3 | 1/3 | NA | |
| 7 | 4 | 2 | 1 | 4 | 0 | 0 | 2 | 0 | 4 | 0 | 2 | 2/3 | 3/3 | 3/3 | 3/3 | 3/3 | 1/3 | NA | |
| H1N1/H5N1 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | NA |
| 4 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0/2 | 0/2 | 0 | 0 | 0 | 0 | 0 | 0 | NA | |
| 6 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | NA | |
| 18 | 14 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 5 | 12 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | NA | |
| H3N2/H5N1 | 2 | 4 | 1 | 1 | 3 | 0 | 0 | 2 | 1 | 3 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 4 | 5 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 5 | 1 | 5 | 3 | 3 | 1 | 3 | 2 | 1 | 1 | |
| 6 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 2 | 1 | 2 | 2 | 0 | 2 | 0 | |
| 7 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | NA | |
a
Abbreviations: ISH, in situ hybridization; NA, not available.
b
Viral infection groups.
c
Number of days postinfection that the animals were sacrificed.
d
Number of animals studied in each group at each time point.
e
See Table S2 in the supplemental material for data on individual animals.