FIG 6.

Translocation of IRF3 into IBs in the presence of NSs and TBK1. (A to C) HeLa cells were transfected with Flag-IRF3 or with Flag-IRF3 and NSs with or without TBK1, and the cells were fixed and permeabilized, followed by staining with anti-Flag and/or anti-HA antibodies as well as FITC- or TRITC-conjugated secondary antibodies. The stained cells were subsequently subjected to confocal microscopy. (A) Cells were transfected with Flag-IRF3, which was found in the cytoplasm. (B) Cells were cotransfected with Flag-IRF3 and HA-NSs. While NSs was in IBs, IRF3 dispersed in the cytoplasm. (C) Cells were cotransfected with Flag-IRF3, HA-NSs, and nontagged TBK1. IRF3 was colocalized in IBs with NSs.