FIG 5.
Accumulation of CD8 T cells and B cells but not CD4 T cells is impaired in IL-17A-deficient mice with persistent cryptococcal lung infection. WT C57BL/6 mice and IL-17A−/− mice were infected by the intratracheal route with C. neoformans strain 52D. At weeks 0 (uninfected), 1, 2, 4, and 8, lungs were removed and enzymatically digested, and lung cells were antibody stained and analyzed by flow cytometric analysis to identify the number of CD4 T cells (top panel), CD8 T cells (middle panel), and B cells (bottom panel) as described in Materials and Methods. Black bars, WT mice; white bars, IL-17A−/− mice. Data are means ± standard errors obtained from 4 to 6 mice per time point in two separate experiments. *, P < 0.05 by ANOVA with Dunnett's post hoc analysis versus day 0 (uninfected) mice of the same strain; ‡, P < 0.05 by unpaired Student t test between C57BL/6 mice and IL-17A−/− mice at the same time point.