FIG 4.

Dectin-2 is required during systemic C. glabrata infection for subsequent splenocyte recall responses. Wild-type or Dectin-2^−/− mice received 10⁷ CFU of C. glabrata per mouse intravenously. (A) After 3, 7, and 14 days, peritoneal macrophages were restimulated ex vivo with heat-killed C. glabrata (Cg), heat-killed C. albicans yeast (Cay), or hyphae (Cah) for 24 h. Levels of TNF-α accumulation in the supernatants were measured by ELISA. Results are means ± SEM (n = 9 or 10 mice per group) from 2 independent experiments. Significance was determined with the Mann-Whitney U test. Statistically different groups are indicated as follows: *, P < 0.05. (B and C) Wild-type or Dectin-2^−/− mice received 10⁷ CFU of C. glabrata per mouse intravenously. After 3, 7, and 14 days, splenocytes were restimulated ex vivo with either heat-killed C. glabrata (B) or heat-killed C. albicans yeast (C) for 48 h and 5 days. Levels of accumulation of TNF-α, IL-10, and IFN-γ (48 h) and of IL-17 and IL-22 (5 days) in the supernatants were measured by ELISA. Results are means ± SEM (n = 14 or 15 mice per group) from 3 independent experiments. Significance was determined with the Mann-Whitney U test. Statistically different groups are indicated as follows: *, P < 0.05; **, P < 0.01; ***, P < 0.001.