FIG 6.

Impact of Dectin-2 deficiency on phagocytosis and killing of C. glabrata by neutrophils. (A) Phagocytosis and killing of C. glabrata cells by murine neutrophils. The results are represented as percentage of phagocytosis (the percentage of fungal cells engulfed by neutrophils present in the well) and percentage of killing (the percentage of killed fungal cells among the phagocytosed yeast). The C. glabrata/neutrophil ratio was 10:1. Values represent means ± SEM (n = 10 mice per group) for 2 independent experiments. P values are for phagocytosis or killing induced by wild-type and Dectin-2^−/− macrophages. *, P < 0.05. (B) Dectin-2^−/− neutrophils cannot induce the respiratory burst to the same extent as the wild type. Dectin-2^−/− or wild-type neutrophils were stimulated with RPMI, phorbol 12-myristate 13-acetate (PMA), live opsonized C. glabrata, or live opsonized C. albicans, and overall oxygen radical production (area under the curve after 60 min) was measured with luminol-enhanced chemiluminescence in isolated neutrophils. Data are represented as means ± SEM (n = 10 mice per group) for 2 independent experiments. Significance was determined with the Mann-Whitney U test. Statistically different groups are indicated as follows: *, P < 0.05; ***, P < 0.001.