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Culture supernatant protein profiles of wild-type B. bronchiseptica strain KM22 and isogenic mutant KM22ΔbscN. Secreted proteins were recovered from bacterial culture supernatants by precipitation with trichloroacetic acid, separated by SDS-PAGE, and visualized by silver staining. Arrowheads indicate proteins present in the supernatant from KM22 and not in supernatant from KM22ΔbscN. The positions of standard proteins and their molecular masses in kilodaltons are indicated on the right.
