TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant genotype or description | Reference or source |
|---|---|---|
| E. coli strains | ||
| DH5 | hsdR17 recA1 gyrA endA1 relA1 | 56 |
| C41(DE3) | F− ompT gal dcm hsdSB(rB− mB−) (DE3) | 57 |
| S. pyogenes strains | ||
| MGAS5005 | M1T1 covS | 29 |
| 5448 | M1T1 | 58 |
| 5448AP | M1T1 covS | 58 |
| MGAS5005.ΔptsI | ΔptsI mutant in strain MGAS5005 | This study |
| MGAS5005.ΔptsIc | ΔptsI complemented with pKSM456 insertion | This study |
| 5448.ΔptsI | ΔptsI mutant in strain 5448 | This study |
| 5448AP.ΔptsI | ΔptsI mutant in strain 5448AP | This study |
| Plasmids | ||
| pCRK | Temperature-sensitive conditional vector; Kmr | 59 |
| pBluescript II KS(−) | ColE1 ori Ampr lacZa | Stratagene |
| pSL60-1 | Vector containing nonpolar aad9 gene | 33 |
| pKSM645 | ΔptsI mutagenic plasmid; nonpolar aad9 | 3 |
| pBlue.ΔptsI | ΔptsI in pBluescript II KS(−); bla (Apr) | 3 |
| pKSM456 | Promoterless ptsI integration plasmid; Kmr | This study |