TABLE 2.
Primers used in this study
| Target gene | PCR primer | Sequence (5′–3′)a | Reference |
|---|---|---|---|
| ptsI | ptsI-1a | agatctGCTGAGTGACTTGTACGA | This study |
| ptsI-1b | GGTATTCATGCGCGTCCA | This study | |
| ptsI-2a | gcgGCCTTGTGTTGGTGGTTTAAGAGCAAC | This study | |
| ptsI-2b | GTCACTCAGCagatctCGTGCGCTTACAGAATGT | This study | |
| ptsI-3a | tctagaGCGGCCTTGTGTTGGTGGTTT | This study | |
| ptsI-3b | ctcgagGGTATTCATGCGCGTCCA | This study | |
| ptsl-internal sense | CAAATTGGTCTGCAAGC | This study | |
| ptsl-internal antisense | CAGATACTGCTCAACTTAACA | This study | |
| ptsl-external sense | GCTAGCAAAAAAGAGCTGGTTTA | This study | |
| ptsl-external antisense | CTCTTGACTACAAAGGTAAAGCAGTAAA | This study | |
| 5005.645jxn-sense | GCGGGTTATTTTTTAAATGTTTCCGAAG | This study | |
| 5005.645jxn-antisense | GGGCCATCTGCAAAATACAAAGCAT | This study | |
| ptsIcomplementL | cccggatccCATCACTCTTGACTACAA | This study | |
| ptsIcomplementR | cccggatccTTAATCTTCAGAAACGTA | ||
| ptsI M1 RT L | CGGAAACCAAGGAATGGAT | This study | |
| ptsI M1 RT R | TGGCAAACCTGTTGTGGTT | ||
| aad9 | aad9L2-bglII | gcgcagatctGGGTGACTAAATAGTGAGGAG | 19 |
| aad9R2-bglII | gcgcagatctGGCATGTGATTTTCC | ||
| gyrA | gyrA M1 RT L | CGACTTGTCTGAACGCCAAAGT | 60 |
| gyrA M1 RT R | ATCACGTTCCAAACCAGTCAAAC | ||
| sagA | sagA M1 RT L | GCTACTAGTGTAGCTGAAACAACTCAA | 19 |
| sagA M1 RT R | AGCAACAAGTAGTACAGCAGCAA |
a
Lowercase letters indicate nucleotides not complementary to the target DNA; underlining indicates restriction sites. Italic nucleotides are in the overlap region for SOEing.