FIG 2.

Organization of the yce tellurite resistance gene cluster in B. anthracis. (A) Organization of the tellurite resistance gene cluster in B. anthracis with number of amino acids (aa) listed below each gene. The upward arrow indicates the site of transposon and plasmid insertion for the transposon mutant 1 and the insertional mutant 1 (TN1/IM1). Leftward arrows indicate primers used for reverse transcription (RT 1, RT 2, or RT 3). Arrowheads show location of PCR pairs A to G. The bent arrow indicates the predicted promoter region. (B) PCR using cDNA from indicated RT primers. Letters correlate with primer combinations shown in panel A. (C) Semiquantitative RT-PCR expression of the yceC, yceG, and yceH genes from cDNA prepared from WT, TN1, IM1, or TN1 complemented with an expression plasmid containing yceG and yceH (pGH).