TABLE 1.
Summary of attenuated mutants identified in C. elegans infection screen
| Mutant | Disrupted genea | Gene symbol | Sequence surrounding insertion siteb |
|---|---|---|---|
| TN1 | Putative tellurite resistance gene | BAS0389 | ACGTATCATTTGGTATGGTGATGCACAGGAAAGTCGTATATATTTCTTATATTTTCTTATTATGCTCGGTTGTGATGTGCTTTATTATCACCCAGAAGGA |
| TN2 | BNR repeat domain protein | BAS2814 | AACCAAATCTATCAGTTCGCATTTGTAAAGAAGTAAATTGAATATCATTATTTTCCAATATATCACCTCATTCTTATCATATATATATTCGTATGCTATT |
| TN3 | Conserved hypothetical protein | BAS0642 | GCACTTGTAAAAGATAAAACACAAGTGAAAATTCTTGTTTCACCTAAATAATATAGAATAGTAAAAAGTAGCTCTTTTCTAGAAATAGAAGAGGGCTACT |
| TN4 | Protease production regulatory protein | BAS0976 | TGCATAAAAACACGGCATATCTATGTGATATGCCGTGTCAAGGTCAATTTATATAAAAAATATTTGTTATATGCAAAAAACTAAGCAGGAATTTGTTTAC |
| TN5 | Hypothetical protein | BAS0432 | ATTGTAAAAATTGCGACCATTCAACTGATCCAGGACATTATATGTAAGTAAAAAAATAAGGGGATGGAAATCATGAGAGAACGTGAAAATTGGGATGTAT |
| TN6 | Spore germination protein | BAS3369 | GTATTAATAGGACCTATGACCTTTTCATCCTTTTTTCAAACGATAGATGATTACAGCTTCAGACCAATGATTCCATCTTTTATACGCTTACTTCGTTTCA |
| TN7 | Conserved hypothetical protein | BAS0645 | TAGAGATAGGAGATTCTATCTTGTTTCGCCATAGTAAAGCTGGAGAATTATGTGAGCGGTTTCCTTTTTTATATCGTGTGAAAGAAGGAGAGATTGTTGG |
| TN8 | Sensor histidine kinase | BAS0869 | GAGAGGAAAAGAATGTTTCCTTCTTTGTGACGGATATTGGGCACGATATTGTATTTGAAGTAATAGATAGAGGGATAGGAATACCGGCAGAAAAAATAAC |
| TN9 | PTS, IIB component | BAS2290 | GTGGTATCATTCCGCCGCAAGCATATACGCCACTTGGCGGACCGACTTTATTAAAGACTGTAAATGATTTAATTCGTTAGGGGGAACCGTTATGAATAAG |
| TN10 | Acetyl-CoA hydrolase | BAS1726 | ATTATTGAGAATTGTGCACATCTAATGTATCGTGATCAACTACGAGCTTATTATGAGGAAGCGAAAACAAGAGGTGGACAAACTCCTCATATTTTAGAGA |
| TN11 | Oxidoreductase | BAS0712 | ATATTTTAGTAAATAATGTCGCGCAGCAATATCCGCAGCAAGGACTAGAGTATATTACAGCAGAACAGTTAGAAAAGACTTTTCGTATTAATATTTTTTC |
a
Abbreviations: PTS, phosphotransferase system; CoA, coenzyme A.
b
Insertion site is shown in bold.