FIG 7.

Inhibition of JAK3 decreases p38 MAPK phosphorylation in BMDMs infected with F. tularensis. (A) BMDMs were inoculated with LVS at an MOI of 200:1. At 0, 15, 30, 60, and 120 min postinoculation, whole-cell lysates were collected and analyzed for total p38 MAPK or p38 MAPK phosphorylated on Thr¹⁸⁰ and Tyr¹⁸² by Western blotting (left). The band intensities (right) were measured in each experiment and were normalized to the loading control (tubulin). The graph presents data from four independent experiments. (B) BMDMs were either untreated or pretreated with 50 μM JANEX-1 (JAK3 inhibitor) for 1 h prior to inoculation with LVS at an MOI of 200:1. At 30 min postinoculation, whole-cell lysates were collected and analyzed for p38 MAPK phosphorylated on Thr¹⁸⁰ and Tyr¹⁸² by Western blotting (left). The band intensities (right) were measured in each experiment and were normalized to the loading control (tubulin). The graph presents data from eight independent experiments. Statistical differences (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001) compared to infected untreated controls were determined by Dunnett's multiple comparison test.