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. 2014 Mar;82(3):1007–1016. doi: 10.1128/IAI.01014-13

FIG 6.

FIG 6

Analysis of the functional domains of Sal0825 in GBS. (A) Diagram of the fragments of Sal0825 expressed from the pNZ8048 vector in GBS 515. (B) Proteins anchored to the bacterial cell wall were isolated and then separated by SDS-PAGE. (C) Western blot analysis with specific antiserum against the epitope tag VSV-G. (D) Relative levels of adherence to human fibronectin among different GBS strains. (B and C) Lanes 1, molecular weight standards (numbers beside the lanes are in thousands); lanes 2, proteins isolated from GBS 515 containing the vector alone (pNZ-SEC); lanes 3 to 7, proteins isolated from 515pNZ-SEC-sal0825 (pNZ-SEC-sal0825), 515pNZ-SEC-AB (pNZ-SEC-AB), 515pNZ-SEC-A (pNZ-SEC-A), 515pNZ-SEC-BC (pNZ-SEC-BC), and 515pNZ-SEC-C (pNZ-SEC-C), respectively. ***, P < 0.001; **, P < 0.01.