FIG 3.

Hemocoel sporozoites retain virulence in mice. In vivo infectivity of sporozoites to C57BL/6 mice by intravenous (continuous lines) and subcutaneous (hatched lines) injections. A total of 5,000 salivary gland sporozoites (black and gray) or hemocoel sporozoites (red and orange) were injected for all experiments. Survival curves are based on cumulative data from three independent experiments (n = 10 each). (A) Kaplan-Meier analysis of the percentage of parasite-free animals over time (days after infection). Infections were monitored daily by microscopic examination of Giemsa-stained blood films. None of the differences were significant (Kruskal-Wallis test). (B) Parasitemias of blood infection. The percentage of parasite-infected erythrocytes is presented on a logarithmic scale. (C) Kaplan-Meier analysis of experimental cerebral malaria (CM) over time (days after sporozoite-induced infections). Analyses by log-rank (Mantel-Cox) test for salivary gland versus hemocoel sporozoites intravenously and subcutaneously were both not significant.