FIG 8.

N. meningitidis induces ErbB4 phosphorylation at site Tyr1284. WCL extracts of MC58-infected HBMEC were analyzed using the pErbB4 (Y1284) antibody. To specify the role of ErbB4 Tyr1284, CHO-K1 cells were transfected with ErbB4 wild type (wt) and the ErbB4 Y1284F point mutant. At 24 h posttransfection, cells were infected with N. meningitidis, and percentages of adhesive (black bars) and invasive (gray bars) bacteria were determined. *, P < 0.05, relative to cells transfected with ErbB4 wt. Western blot analysis demonstrated comparable expression levels of ErbB4 and ErbB4 Y1284F.