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. 2014 Mar;82(3):1052–1063. doi: 10.1128/IAI.01378-13

FIG 2.

FIG 2

Modulation of T cell responses. OVA-specific serum IgE (A), IgG1 (B), and IgG2a (C) levels were quantified from individual serum samples by ELISA. Histograms represent means, and error bars represent SD of titers from individual mice. Cell-free supernatants of OVA-restimulated mediastinal lymph node cells were analyzed for IL-4 (D), IL-5 (E), IL-13 (F), IFN-γ (G), and IL-10 (I) by ELISA using matched antibody pairs. Histograms represent means, and error bars represent SD of ELISA replicates performed on three individual stimulations from each group. (H) Representative FACS plot of splenocytes analyzed for the frequency of IFN-γ-producing CD4+ and CD8+ T cells on the day of analysis. The plots are gated on live CD3+ lymphocytes. The FACS plots are representative plots from two individual experiments with 4 to 6 mice per group. Data are represented as means plus SD and are representative of three individual experiments with 3 to 6 mice per group. An ANOVA test was used to determine statistical significance. n.s, nonsignificant; **, P < 0.01.