Runx3-regulated genes in NKC. (A) Heat map showing Runx3−/−/WT fold expression (log 2 ratio) for a subset of Runx3-regulated genes in in vivo IL-15-activated iNKC (CD27, CD27+ CD11b−) and mNKC (CD11b, CD11b+ CD27−). (B) qRT-PCR analysis of Runx3-regulated genes in in vivo IL-15-activated spleen iNK (CD27+ CD11b−) and mNK (CD11b+ CD27−) cell populations. (C) FACS analysis of Runx3-regulated genes: Kit expression in iNKC (CD27+ CD11b−) 24 h after the second IL-15/Rα injection and CD226, CD81, Cxcr3, and Itgαx in spleen NKC 48 h after the second IL-15/Rα injection.