FIG 1.

Generation of Jmjd2c knockout ESCs. (A) Overview of the Jmjd2c locus with the location of loxP sites (arrows) indicated relative to exon positions (squares). The domain structure of Jmjd2c is shown below. (B) Agarose gels showing PCR amplification of Jmjd2c floxed (∼1,200 bp) and deletion (∼400 bp) alleles for ESCs homozygous or heterozygous for the conditional Jmjd2c allele grown in the absence or presence of OHT to induce Cre recombination. (C) Relative Jmjd2c mRNA levels in control (−) and OHT-treated (+OHT) Jmjd2c knockout ESCs determined by quantitative RT-PCR analyses with primers covering different exons. Transcript levels were normalized to Rplp0, and error bars show standard deviations for technical replicates. (D) Immunoblots of conditional Jmjd2c knockout ESCs probed with an antibody raised against an internal peptide of Jmjd2c (α-Jmjd2c K) or antibodies recognizing Jmjd2a, Jmjd2b, or vinculin (loading control).