FIG 6.

The double Tudor domain of Jmjd2c recognizes H3K4me2/me3 and is required for binding to target genes. (A) Biotin-labeled histone H3 or histone H4 peptides were used in IP experiments with the indicated GST fusion proteins. (B) Schematic representation of the domain structure of wild-type (wt) Jmjd2c and the generated mutants lacking PHD and/or double Tudor domains. (C to F) Conditional Jmjd2c knockout MEFs were transduced with an empty vector or vectors encoding wt or Jmjd2c mutants, treated with OHT or left untreated as indicated, and analyzed by WB (C and E) and ChIP (D and F). (G and H) MEFs transduced with shRNA targeting Dpy30 or a scrambled control were analyzed by WB (G) and used for ChIP experiments (H). For histone marks, ChIP signals were normalized to H3 ChIP values.