FIG 7.

Depletion of JMJD2C in KYSE150 cells affects expression of a subset of target genes. (A) Western blot of KYSE150 cells transfected with a control scrambled shRNA (shCtrl) or shRNAs targeting JMJD2C and/or JMJD2A. (B) Bar graph showing the percentage of down- and upregulated genes, which were identified as containing a JMJD2C binding site within 1 kb of a TSS in the ChIP-seq analyses. JMJD2C binding sites were more frequently found at down- or upregulated genes compared to all genes present on the array (one-sided Fischer's exact test; P = 7.21E−55 and P = 2.34E−13, respectively). (C) RT-qPCR validation of selected targets. The gene expression analysis identified BUB1B and OPA1 as downregulated in the JMJD2C- but not JMJD2A-depleted samples, while TGFB2 was induced under both conditions. (D) GO analysis of JMJD2C-bound and downregulated genes.