FIG 4.

Subcellular distribution of the CSN complex is not affected by the cell cycle. Cells were synchronized and collected at different stages of the cell cycle, as indicated. Cellular proteins were then fractionated in the absence of phosphatase inhibitors and probed with anti-CSN and anti-cyclin E antibodies, as indicated. Cell cycle stages were verified by flow cytometry, as displayed in the plots above the lanes. Nonsynchronized cells (C) were used as a control.