FIG 5.

CSN subunits are transiently recruited to the nucleus following UV damage, in a dose-dependent manner. (A) Cells were exposed to 20 J/m² of UV-C irradiation. Cellular proteins were fractionated into cytosolic (Cyt), nucleoplasmic (N), and chromatin (Chr) fractions, at different time points following DNA damage induction, and monitored by Western blot analyses using anti-CSN antibodies, as indicated. In CSN6, the thin dashed line represents an additional time point that was omitted. (B) Cells were treated with increasing doses of UV-C light, fractionated after 10 min, and probed with various antibodies, as indicated. As a control for DNA damage-dependent recruitment to chromatin, we used DDB2; histone H3 was used as a loading control.