FIG 3.

Histopathological changes in brain after VSV infection. Mice were euthanized at day 7 postinoculation. A horizontal brain section containing the olfactory bulb, forebrain, cerebrum, hippocampus, and cerebellum was fixed in 4% (vol/vol) phosphate-buffered paraformaldehyde, followed by HE straining. Detailed criteria for evaluation of brain histological change are described in Materials and Methods. DMEM control, grade 0 (no lesion); rVSV-K1651A, grade 1 (mild); rVSV-G4A, grade 1 (mild); and rVSV, grade 3 (severe).