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Quantification of IFN-β mRNA by real-time RT-PCR. Mice were infected with rVSV, rVSV-G4A, rVSV-K1651A, and rVSV-G1670A at dose of 5 × 106 PFU/mouse. At days 3 postinfection, lung and brain tissues were collected. Total RNA was extracted from lung or brain tissues and was treated with DNase I (Ambion). The IFN-β mRNA in each tissue was quantified by real-time RT-PCR. The average amounts of IFN-β mRNA from 5 mice for each group are shown.
