FIG 7.
Control of type I and II IFN responses in PBMCs. (A to C) Levels of IFN-α (A), IFN-β (B), andIFN-γ (C) mRNA expression in PBMCs of animals infected with the parental strain 5804PeH (WT), the Vko derivative, or the different mutant viruses. RNA was isolated from PBMCs on day 0 and 3 postinfection, and PCR assays were conducted using primers specific for the genes indicated at the top of each panel (IFN-α, IFN-β, and IFN-γ). The fold change ratios between experimental and control samples for each gene were calculated and normalized against GAPDH using the ΔΔCT method. Error bars represent the SEM. Asterisks show the levels of statistical significance as follows: **, P < 0.01; ***, P < 0.001.