FIG 4.

Colchicine and vinblastine treatment of Neuro2a cells reduces the growth of RSA59 but not RSMHV2. At 24 h posttreatment with colchicine and vinblastine, cells were infected with RSA59 or RSMHV2 at 0.5 MOI. Culture supernatant was collected for viral titer estimation, and viability of infected cells was measured by the MTT assay. Viral titers were normalized based on the live cell numbers measured by the MTT assay, and titers were plotted for RSA59 (A)- and RSMHV2 (B)-infected cultures. Mean and standard errors of the means (SEM) of titers from 4 wells in one representative experiment (of three) are shown.