FIG 1.

Characterization of WSSV miRNAs. (A) Sequence alignments of WSSV miRNAs and known viral miRNAs. MGHV, mouse gammaherpesvirus; HSV2, herpes simplex virus 2; MCMV, mouse cytomegalovirus; HVT, herpesvirus of turkey. (B) Sequence alignments of WSSV miRNAs and shrimp miRNAs. (C) Time course expression patterns of WSSV miRNAs in WSSV-infected shrimp at different times postinfection. The lane headings show the time points postinfection. The numbers on the right indicate the log numbers of miRNA copies. (D) Northern blots of WSSV miRNAs. Total RNAs isolated from lymphoid organs of virus-free and WSSV-infected shrimp at different times postinfection were blotted with DIG-labeled oligodeoxynucleotide probes. The RNA sizes are shown to the right of the blots. U6 was used as a loading control. Lane headings show the time points postinfection.