FIG 6.

HCV NS2 and NS5B inhibit MICA/B expression in Huh7.5 cells in a coculture with NK cells. (A) MICA/B expression in HCV genomic region-transfected Huh7.5 cells was analyzed by Western blotting following coculture with rIL-2-activated NK cells. Stable transfectants with empty vector, core, nonstructural proteins (BB7), or E1/E2/p7 were used. (B) Huh7.5 cells transiently transfected with NS2, NS3/4A, NS5A, or NS5B were cocultured with rIL-2-activated NK cells, and MICA/B expression in Huh7.5 cells was analyzed by Western blotting. Cellular actin was used for comparison of protein loads in each experiment.