FIG 7.

Liposome binding assay and biosensor analysis. Liposomes are injected into the L1 chip to saturation (∼8,000 RU) (not shown), and soluble gB(730t) is then injected at 5 μl/min for 240 s, followed by the indicated antibody (240 s); binding to liposomes is measured as an increase in RU. There is some day-to-day variability in the RU of gB captured on the liposomes (y axis values), because different batches of liposomes and different preparations of gB were used. However, the concentrations of liposomes and gB were always held constant, and the test samples were always compared to a control (IgG or Fab flowed across liposomes only) on the same day. The beginning of each injection is indicated with an arrow, and arrowheads along the x axis show where each injection was stopped. (A and B) Competition for binding to gB between MAbs SS55 and SS144. SS10, SS55, and SS144 were injected at a concentration of 75 μg/ml. (C) DL16 was injected at a concentration of 50 μg/ml. The double slash in the x axis indicates a pause before the injection of DL16.